RNA berevolusi membentuk DNA untuk melindungi kode genetik dari kerusakan karena bentuk rantainya yang beruntai ganda. ![]() Beberapa ilmuwan percaya, jika RNA berevolusi membentuk DNA karena struktur RNA yang lebih sederhana. ![]() ![]() This efficient approach greatly expands the repertoire of RNA editing targets and can be applied to studies involving RNA editing-related human diseases. Kadar DNA tidak dipengaruhi oleh aktivitas sintesis protein, sedangkan RNA dipengaruhi. Specificity of our profiling was supported by observations of enrichment with known features of targets of adenosine deaminases acting on RNA (ADAR) and validation by means of capillary sequencing. A comprehensive set of several hundred human RNA editing sites was detected by comparing genomic DNA with RNAs from seven tissues of a single individual. We developed an unbiased assay to screen more than 36,000 computationally predicted nonrepetitive A-to-I sites using massively parallel target capture and DNA sequencing. To date, only a handful of functional sites have been identified in mammals. Further, if the A-to-I DNA editing at RNA:DNA hybrids were the sole cause of A-to-I (read as A-to-G) mutation events for Ig SHM in vivo then the exact opposite strand biases at A:T base pairs (T > A) of what is. ![]() N2 - Adenosine-to-inosine (A-to-I) RNA editing leads to transcriptome diversity and is important for normal brain function. The RNA moiety of RNA:DNA hybrids is also edited at similar lower frequencies relative to the editing rate at dsRNA substrates. T1 - Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencing.
0 Comments
Leave a Reply. |